Cat.# 0005

Pseudouridine (Ψ) is a post-transcriptional RNA modification that alters RNA–RNA and RNA–protein interactions that affect gene expression¹. It is demonstrated that Ψ can enhance RNA stability and decrease anti-RNA immune response, it has been employed for developing effective mRNA vaccines against COVID-19 during the pandemic and leads to the researchers won The Nobel Prize in Physiology or Medicine 2023².

The Norgene Rapid Ψ RNA Enrichment Kit is simple and rapid workflow to enable identification and profiling of pseudouridine (Ψ) RNAs at transcriptome-wide level. Suitable for downstream RNA library construction and sequencing or qRT-PCR to check pseudouridine (Ψ) RNA transcripts.

HIGHLIGHTS

• Simple workflow: From beginning to end the protocol can be completed in just one day.
• Accurate and reproducible: Innovative immunoprecipitation and elution technology ensure efficient and reproducible results across replicates.

References

1. Erin K. Borchardt,* Nicole M. Martinez,* and Wendy V. Gilbert, Regulation and Function of RNA Pseudouridylation in Human Cells, Annual Review of Genetics, 2020

2. Anderson, B.R., et al., Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation. Nucleic Acids Res. 2010