Cat.# 0011

N1-methyladenosine (m1A) is a common and reversible post-transcriptional modification found in tRNA, rRNA, and mRNA. Recent advancements in analytical chemistry and high-throughput sequencing technologies have uncovered its essential roles in gene regulation and various biological Processes^1,2.

The Norgene rapid m1A RNA Enrichment Kit is simple and rapid workflow to enable identification and profiling of m1A (N1-methyladenosine) RNA methylation sites at transcriptome-wide level. Suitable for downstream RNA library construction and sequencing or qRT-PCR to check methylated RNA transcripts. 

HIGHLIGHTS

• Simple workflow: From beginning to end the protocol can be completed in just one day.
• Accurate and reproducible: Innovative immunoprecipitation and elution technology ensure efficient and reproducible results across replicates.

References

1. Xiaoyu Li, Xushen Xiong, Kun Wang, Lixia Wang, Xiaoting Shu, Shiqing Ma & Chengqi Yi. Transcriptome-wide mapping reveals reversible and dynamic N1-methyladenosine methylome, Nature Chemical Biology volume 12, pages311–316 (2016)

2. Yang W, Meng J, Liu J, Ding B, Tan T, Wei Q, et al. The N(1)-methyladenosine methylome of petunia mRNA. Plant Physiol. (2020);183:1710–24